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Cytotoxicity testing

In the context of its in vitro biocompatibility concept MicroMol offers the analysis of chemicals, cosmetics, substances, medical devices and technical material according to their cytotoxic potential according to the ISO DIS 10933-5 in well established cell culture systems. Moreover, in addition to the normative confirmed platform we offer the performance of  your material on primary human leukocytes giving an extremely high level of sensitivity.
According to its complexity and chemistry we will confront the cells either directly with the test material (solid or liquid) or with hydrophillic or lipophillic extracts thereof. Based on profound chemical expertise we will help the customer to define the correct testing modules for its test samples. Finally MicroMols cytotoxicity concept will also help you to classify your chemicals and substances within the REACH initiative of the EU.

Expert: Wolfgang Rudy (www.micromol.com)

Skin corrosivity

The second branch of MicroMol´s biocompatibility platform addresses the analysis of chemicals, cosmetics, substances, medical devices and technical material according to their corrosive potential on a reconstituted human epidermis model (SkinEticTM RhE system). The 3D RhE model consists of human keratinocytes grown on a membrane. This culture method induces the cells to grow in multilayers and to form junctions so that the cultures are similar to mini pieces of human skin. The RhE is confronted with the test material, either solid or liquid, for at least two different time intervals ( 3  min, 1 hour) with subsequent determination of cell viability giving the customer profound information on localized distructive effects upon topic administration of the test material. The SkinEthicTM RhE test method underwent a coordinated catch-up study. The method was scientifically validated and found to be sufficiently similar with regard to its structural and functional characteristics and its performance in reference to the Performance Standards as required by OECD TG 431 (ESAC, 2006).

Expert: Wolfgang Rudy (www.micromol.com)

Biocompatibility

MicroMol offers a powerful variant of the SkinEthic corrosivity test, for its ability to predict skin irritation. Using this system the RhE is confronted with the test material for a defined time period followed by a substantial regeneration phase before testing cell damage and viability. The test system was evaluated by the ESAC (2008) and on the basis of the validation study it was concluded that the SkinEthicTM RhE Irritation test  showed evidence of being a reliable and relevant stand alone test for being used as a replacement for the Draize Skin test substances (distinguishing between R38 skin irritating and non-skin irritating; Alépée, et al., 2010; Kandárová, et al., 2009). In 2009, the ESAC substantiates the SkinEthic RHE for testing under the UN GHS.

Expert: Wolfgang Rudy (www.micromol.com)

Transfection

Introduction of your expression vector in an appropriate cell sytem and creation of stable cell lines. We offer a standard panel of high expression lines (SF9, COS, CHO, HEK293) but are also able to switch on every cellular host of your interest.

Expert: Wolfgang Rudy (www.micromol.com)

Expression of recombinant proteins

In addition to the transfection of your cDNA we also express your protein in the desired eukaryotic cell system and isolate positive clones dependent on the expression level. In addion to that we help you to isolate and purify your protein from the cell culture supernatant or from a cell lysate.

Expert: Wolfgang Rudy (www.micromol.com)

Recloning of hybridomas

One of the major problems in hybridoma culture and antibody production is the overgrowth of an antibody producing culture by non producers. We reclone your hybridoma and provide you with a panel of clonal producers of your desired antibody.

Expert: Andreas Dreusch (http://www.micromol.com)

Production of monoclonal antibodies in Bioreactors

Antibody production is a time and space consuming step for every competitive lab. We offer the possibilty to grow your hybridoma in a mid scale bioreactor with the possibilty to produce up to 50 mg Monoclonal/month. We can provide you with high titer supernatants and – of course – with the purified and evaluated antibody.

Expert: Wolfgang Rudy (www.micromol.com)

Isolation of cell subpopulations by magnetic cell sorting

MicroMol offers the possibility to isolate and characterize cell subpopulations by magnetic cell sorting according to the expression of a defined and specific cell surface marker. This might be a transfectant expressing a certain cell membrane protein or even leucocyte subpopulations according to the expression of CD3, CD4, CD8, CD20, CD56 or other designation markers.

Expert: Wolfgang Rudy (www.micromol.com)

Generation of cell lines from primary material

The generation of cell lines from primary material and the establishment of special culture conditions therefore is a tedious and time consuming step for every competitive lab. With sophisticated experience in tissue culture MicroMol will do that for you and help you to save your time for important experiments.

Expert: Wolfgang Rudy (www.micromol.com)