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Cytotoxicity testing

Analysis of chemicals, substances and material according to their toxic potential in a well established cell culture system. In addition to the standard test MicroMol offers the possibilty to enhance the sensitivity by testing your material on primary human leukocytes. MicroMols Cytotoxicity concept will also help you to classify your chemicals and substances within the REACH initiative of the EU.

Expert: Andreas Dreusch (http://www.micromol.com)

Transfection

Introduction of your expression vector in an appropriate cell sytem and creation of stable cell lines. We offer a standard panel of high expression lines (SF9, COS, CHO, HEK293) but are also able to switch on every cellular host of your interest.

Expert: Wolfgang Rudy (www.micromol.com)

Expression of recombinant proteins

In addition to the transfection of your cDNA we also express your protein in the desired eukaryotic cell system and isolate positive clones dependent on the expression level. In addion to that we help you to isolate and purify your protein from the cell culture supernatant or from a cell lysate.

Expert: Wolfgang Rudy (www.micromol.com)

Recloning of hybridomas

One of the major problems in hybridoma culture and antibody production is the overgrowth of an antibody producing culture by non producers. We reclone your hybridoma and provide you with a panel of clonal producers of your desired antibody.

Expert: Andreas Dreusch (http://www.micromol.com)

Production of monoclonal antibodies in Bioreactors

Antibody production is a time and space consuming step for every competitive lab. We offer the possibilty to grow your hybridoma in a mid scale bioreactor with the possibilty to produce up to 50 mg Monoclonal/month. We can provide you with high titer supernatants and – of course – with the purified and evaluated antibody.

Expert: Wolfgang Rudy (www.micromol.com)

Isolation of cell subpopulations by magnetic cell sorting

MicroMol offers the possibility to isolate and characterize cell subpopulations by magnetic cell sorting according to the expression of a defined and specific cell surface marker. This might be a transfectant expressing a certain cell membrane protein or even leucocyte subpopulations according to the expression of CD3, CD4, CD8, CD20, CD56 or other designation markers.

Expert: Wolfgang Rudy (www.micromol.com)

Generation of cell lines from primary material

The generation of cell lines from primary material and the establishment of special culture conditions therefore is a tedious and time consuming step for every competitive lab. With sophisticated experience in tissue culture MicroMol will do that for you and help you to save your time for important experiments.

Expert: Wolfgang Rudy (www.micromol.com)