Cell Biology and Tissue Culture
10 years experience in tissue culture technology makes MicroMol to an outstanding partner in advanced cell culture technology. One of the platform technologies in this context is the analysis of the cytotoxic potential of chemicals, reagents and substances in defined cell culture settings, even with primary human leukocytes. In addition to that MicroMol will help you to express your protein in an eukaryotic cell line, to create transfectans with your expression vector, to produce an defined antibody or even to create a cell line from primary material.
“The fine art of life science” will be your optimal partner in every question concerning your tissue culture needs and problems.
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Cytotoxicity testing
Analysis of chemicals, substances and material according to their toxic potential in a well established cell culture system. In addition to the standard test MicroMol offers the possibilty to enhance the sensitivity by testing your material on primary human leukocytes. MicroMols Cytotoxicity concept will also help you to classify your chemicals and substances within the REACH initiative of the EU.
- Transfection
Introduction of your expression vector in an appropriate cell sytem and creation of stable cell lines. We offer a standard panel of high expression lines (SF9, COS, CHO, HEK293) but are also able to switch on every cellular host of your interest.
- Expression of recombinant proteins
In addition to the transfection of your cDNA we also express your protein in the desired eukaryotic cell system and isolate positive clones dependent on the expression level. In addion to that we help you to isolate and purify your protein from the cell culture supernatant or from a cell lysate.
- Recloning of hybridomas
One of the major problems in hybridoma culture and antibody production is the overgrowth of an antibody producing culture by non producers. We reclone your hybridoma and provide you with a panel of clonal producers of your desired antibody.
- Production of monoclonal antibodies in Bioreactors
Antibody production is a time and space consuming step for every competitive lab. We offer the possibilty to grow your hybridoma in a mid scale bioreactor with the possibilty to produce up to 50 mg Monoclonal/month. We can provide you with high titer supernatants and – of course – with the purified and evaluated antibody.
- Isolation of cell subpopulations by magnetic cell sorting
MicroMol offers the possibility to isolate and characterize cell subpopulations by magnetic cell sorting according to the expression of a defined and specific cell surface marker. This might be a transfectant expressing a certain cell membrane protein or even leucocyte subpopulations according to the expression of CD3, CD4, CD8, CD20, CD56 or other designation markers.
- Generation of cell lines from primary material
The generation of cell lines from primary material and the establishment of special culture conditions therefore is a tedious and time consuming step for every competitive lab. With sophisticated experience in tissue culture MicroMol will do that for you and help you to save your time for important experiments.
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