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BioReference W3110 kit, anti-W3110-antibody, W3110 whole cell extract
(plus 19% VAT: 14,25 EUR)
E. coli W3110 (DSM 5911, ATCC 27325) is still the workhorse for many recombinantly expressed proteins for pharmaceutical purposes.
The strain has a well documented pedigree and is one of the most unmodified K12 strains. It was derived from a K12 wild-type by UV-irradiation and two spontaneus mutations. It is F- and lambda-. On the other hand it is easily detected by a large genomic inversion. The strain is often modified for the specific demands of the expression system.
The BioRefernce W3110 kit is well suited for the detection of residual proteins from the W3110 host strain in your purified protein preparation. The antibody reacts to a big extent with other E. coli K12 strains, but you might miss the proteins that were introduced by the construction of many typical strains used for cloning. The antibody can not detect proteins from the F-plasmid since the strain is plasmid free.
Package content: 100 µg antibody + 20 µg protein, instruction leaflet
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Empty basket!BioReference W3110 kit, anti-W3110-antibody, W3110 whole cell extract
(plus 19% VAT: 14,25 EUR)
E. coli W3110 (DSM 5911, ATCC 27325) is still the workhorse for many recombinantly expressed proteins for pharmaceutical purposes.
The strain has a well documented pedigree and is one of the most unmodified K12 strains. It was derived from a K12 wild-type by UV-irradiation and two spontaneus mutations. It is F- and lambda-. On the other hand it is easily detected by a large genomic inversion. The strain is often modified for the specific demands of the expression system.
The BioRefernce W3110 kit is well suited for the detection of residual proteins from the W3110 host strain in your purified protein preparation. The antibody reacts to a big extent with other E. coli K12 strains, but you might miss the proteins that were introduced by the construction of many typical strains used for cloning. The antibody can not detect proteins from the F-plasmid since the strain is plasmid free.
Package content: 100 µg antibody + 20 µg protein, instruction leaflet