Protein Expression / Antibodies

BL21 Kit


 With the BL21 Kit you can easily detect residual contaminants from the BL21 (DE3) host strain in your purified protein preparation.
The kit contains the standardized BL21 Whole Cell Extract (1 µg/µl) and the highly specific and sensitive rabbit-derived BL21 antiserum Anti-BL21 Serum.

Info Box: BL21 (DE3)
BL21 (DE3) is the most often used host strain for protein expression with the T7 polymerase / prophage DE3 system of Studier and Moffatt (1985). It was derived from the Lon protease deficient BL21 B-type E. coli strain (Daegelen, Studier, Lenski, Cure, Kim: "Tracing ancestors and relatives of Escherichia coli B, and the derivation of B strains REL606 and BL21(DE3)"; J. Mol. Biol., Vol. 394/4(2009): 634-643).  Its genotype is F- ompT hsdSB (r-B m-B) gal dcm (DE3). The strains are valuable for research and the fast establishment of a production and purification procedure.


It.No. Content Unit Size Price*:   
321 BL21 Kit 1 kit (100 µl antiserum, 400 µl cell extract) 320,00 €
Unit Size:
Price*: 320,00 € Quantity:

* All prices are exclusive of VAT and shipping.

Related products


Protein Expression / Antibodies

Detection and Quantification of Recombinant Proteins
MicroMol's BioReference Extracts and Kits are designed for the detection of recombinant proteins and residual proteins from the production strain.
With the GST and HIS kits you can detect and quantify the protein your expression system should produce. The kits contain a reference protein of defined quantity and a ready-to-use anti-tag antibody or conjugate. Tested for ELISA and Western blotting.
The BL21 (DE3), HB101 and W3110 kits are designed for the detection of residual proteins of the expression strain. This is very important for the validation of purification methods. The relevant document rules for pharmaceutical preparations urge the producer of a recombinant protein to confirm that the purification process is able to remove all impurities from the preparation. Our kits are designed to do this by Western blotting or ELISA. They contain raw extracts of the most common production strains and an adequate antiserum that was prepared against whole cell extracts.
Also see the section "Services" for more information.
It.No. Item Unit Size Price*:  Quantity:
322   Anti-BL21 pAb   260,00 €  
569   Anti-BL21 Serum   260,00 €  
323   BL21 Whole Cell Extract   75,00 €  

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