Protein Expression / Antibodies

BL21 Whole Cell Extract


 Whole cell extract from the E. coli strain BL21 (DE3). Especially designed as positive control for the determination of BL21 proteins in purification experiments.
Concentration: 1 µg/µl

Info Box: BL21 (DE3)
BL21 (DE3) is the most often used host strain for protein expression with the T7 polymerase / prophage DE3 system of Studier and Moffatt (1985). It was derived from the Lon protease deficient BL21 B-type E. coli strain (Daegelen, Studier, Lenski, Cure, Kim: "Tracing ancestors and relatives of Escherichia coli B, and the derivation of B strains REL606 and BL21(DE3)"; J. Mol. Biol., Vol. 394/4(2009): 634-643).  Its genotype is F- ompT hsdSB (r-B m-B) gal dcm (DE3). The strains are valuable for research and the fast establishment of a production and purification procedure.


It.No. Content Unit Size Price*:   
323 BL21 Whole Cell Extract 400 µl (1 µg/µl) 75,00 €
Unit Size:
Price*: 75,00 € Quantity:

* All prices are exclusive of VAT and shipping.

Related products


Protein Expression / Antibodies

Detection and Quantification of Recombinant Proteins
MicroMol's BioReference Extracts and Kits are designed for the detection of recombinant proteins and residual proteins from the production strain.
With the GST and HIS kits you can detect and quantify the protein your expression system should produce. The kits contain a reference protein of defined quantity and a ready-to-use anti-tag antibody or conjugate. Tested for ELISA and Western blotting.
The BL21 (DE3), HB101 and W3110 kits are designed for the detection of residual proteins of the expression strain. This is very important for the validation of purification methods. The relevant document rules for pharmaceutical preparations urge the producer of a recombinant protein to confirm that the purification process is able to remove all impurities from the preparation. Our kits are designed to do this by Western blotting or ELISA. They contain raw extracts of the most common production strains and an adequate antiserum that was prepared against whole cell extracts.
Also see the section "Services" for more information.
It.No. Item Unit Size Price*:  Quantity:
322   Anti-BL21 pAb   260,00 €  
569   Anti-BL21 Serum   260,00 €  
321   BL21 Kit   320,00 €  

<- Back
User login

Forgot your password?

Shopping cart is empty
Article Search