Protein Expression / Antibodies

HB101 Kit


 The HB101 Kit helps you to detect contaminations of the host cell in your purified protein by ELISA and/or Western-Blots. Due to the pedigree of HB101, this works with most of the K12 expression hosts.
The kit contains the standardized HB101 Whole Cell Extract (1 µg/µl) as positive control for ELISA and Western-Blot determination as well as the highly specific and sensitive rabbit-derived HB101 antiserum Anti HB101 Serum.

Info Box: HB101
The E. coli K12 strain HB101 (DSM 1607, ATCC 33694)) is one of the grandfathers in molecular biology. It was constructed by complementation analysis of a K12 strain with a B strain. Thus HB101 carries about 10% of the B strain chromosome. Its genotype is F- leuB6 proA2 recA13 thi1 ara14 lacY1 galK2 xyl5 mtl1 rpsL20 supE44 hsdS20 lambda- rB-mB-. HB101 deviates to a big extend from the prototypic K12 strain W3110. On the other hand HB101 was the basis for the construction of many contemporary laboratory strains.


It.No. Content Unit Size Price*:   
315 HB101 Kit 1 kit (100 µl antiserum, 400 µl cell extract) 320,00 €
Unit Size:
Price*: 320,00 € Quantity:

* All prices are exclusive of VAT and shipping.

Related products


Protein Expression / Antibodies

Detection and Quantification of Recombinant Proteins
MicroMol's BioReference Extracts and Kits are designed for the detection of recombinant proteins and residual proteins from the production strain.
With the GST and HIS kits you can detect and quantify the protein your expression system should produce. The kits contain a reference protein of defined quantity and a ready-to-use anti-tag antibody or conjugate. Tested for ELISA and Western blotting.
The BL21 (DE3), HB101 and W3110 kits are designed for the detection of residual proteins of the expression strain. This is very important for the validation of purification methods. The relevant document rules for pharmaceutical preparations urge the producer of a recombinant protein to confirm that the purification process is able to remove all impurities from the preparation. Our kits are designed to do this by Western blotting or ELISA. They contain raw extracts of the most common production strains and an adequate antiserum that was prepared against whole cell extracts.
Also see the section "Services" for more information.
It.No. Item Unit Size Price*:  Quantity:
316   Anti-HB101 Serum   260,00 €  
317   HB101 Whole Cell Extract   75,00 €  

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