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Highly reactive rabbit antiserum directed against the E. coli strain BL21 (DE3). The antibody is a perfect tool to determine bacterial contamination in your BL21 expression experiments.
Info Box: BL21 (DE3) BL21 (DE3) is the most often used host strain for protein expression with the T7 polymerase / prophage DE3 system of Studier and Moffatt (1985). It was derived from the Lon protease deficient BL21 B-type E. coli strain (Daegelen, Studier, Lenski, Cure, Kim: "Tracing ancestors and relatives of Escherichia coli B, and the derivation of B strains REL606 and BL21(DE3)"; J. Mol. Biol., Vol. 394/4(2009): 634-643). Its genotype is F- ompT hsdSB (r-B m-B) gal dcm (DE3). The strains are valuable for research and the fast establishment of a production and purification procedure. |